Colocation and role of polyphosphates and alkaline phosphatase in apatite biomineralization of elasmobranch tesserae

AbstractElasmobranchs (e.g. sharks and rays), like all fishes, grow continuously throughout life. Unlike other vertebrates, their skeletons are primarily cartilaginous, comprising a hyaline cartilage-like core, stiffened by a thin outer array of mineralized, abutting and interconnected tiles called tesserae. Tesserae bear active mineralization fronts at all margins and the tesseral layer is thin enough to section without decalcifying, making this a tractable but largely unexamined system for investigating controlled apatite mineralization, while also offering a potential analog for endochondral ossification. The chemical mechanism for tesserae mineralization has not been described, but has been previously attributed to spherical precursors, and alkaline phosphatase (ALP) activity. Here, we use a variety of techniques to elucidate the involvement of phosphorus-containing precursors in the formation of tesserae at their mineralization fronts. Using Raman spectroscopy, fluorescence microscopy and histological methods, we demonstrate that ALP activity is located with inorganic phosphate polymers (polyP) at the tessera–uncalcified cartilage interface, suggesting a potential mechanism for regulated mineralization: inorganic phosphate (Pi) can be cleaved from polyP by ALP, thus making Pi locally available for apatite biomineralization. The application of exogenous ALP to tissue cross-sections resulted in the disappearance of polyP and the appearance of Pi in uncalcified cartilage adjacent to mineralization fronts. We propose that elasmobranch skeletal cells control apatite biomineralization by biochemically controlling polyP and ALP production, placement and activity. Previous identification of polyP and ALP shown previously in mammalian calcifying cartilage supports the hypothesis that this mechanism may be a general regulating feature in the mineralization of vertebrate skeletons

On the non-paraxial modes of two-dimensional nearly concentric resonators

to be published in Applied OpticsA non-paraxial scalar diffraction integral is used to determine numerically the resonance modes of a two dimensional nearly concentric Fabry-Perot resonator. Numerical examples are provided and results are compared to those published by Laabs and Friberg [IEEE J. Quant. El., vol. 35, pp. 198-207, 1999]. Discrepancies are reported and further discussed on the basis of the difference between the solution space supported by the numerical method used in the present work and the one used by Laabs and Friberg

Effect of Beam Dynamics Processes in the Low Energy Ring ThomX

As part of the R\&D for the 50 MeV ThomX Compton source project, we have studied the effect of several beam dynamics processes on the evolution of the beam in the ring. The processes studied include among others Compton scattering, intrabeam scattering, coherent synchrotron radiation. We have performed extensive simulations of a full injection/extraction cycle (400000 turns). We show how each of these processes degrades the flux of photons produced and how a feedback system contributes to recovering most of the flux.Comment: Submitted to IPAC'14, WEPRO00

Effect of Compton Scattering on the Electron Beam Dynamics at the ATF Damping Ring

Compton scattering provides one of the most promising scheme to obtain polarized positrons for the next generation of $e^-$ -- $e^+$ colliders. Moreover it is an attractive method to produce monochromatic high energy polarized gammas for nuclear applications and X-rays for compact light sources. In this framework a four-mirror Fabry-P\'erot cavity has been installed at the Accelerator Test Facility (ATF - KEK, Tsukuba, Japan) and is used to produce an intense flux of polarized gamma rays by Compton scattering \cite{ipac-mightylaser}. For electrons at the ATF energy (1.28 GeV) Compton scattering may result in a shorter lifetime due to the limited bucket acceptance. We have implemented the effect of Compton scattering on a 2D tracking code with a Monte-Carlo method. This code has been used to study the longitudinal dynamics of the electron beam at the ATF damping ring, in particular the evolution of the energy spread and the bunch length under Compton scattering. The results obtained are presented and discussed. Possible methods to observe the effect of Compton scattering on the ATF beam are proposed

A collimation system for ELI-NP Gamma Beam System - design and simulation of performance

The purpose of this study was to evaluate the performance and refine the design of the collimation system for the gamma radiation source (GBS) currently being realised at ELI-NP facility. The gamma beam, produced by inverse Compton scattering, will provide a tunable average energy in the range between 0.2 and 20 MeV, an energy bandwidth 0.5% and a flux of about 108 photons/s. As a result of the inverse Compton interaction, the energy of the emitted radiation is related to the emission angle, it is maximum in the backscattering direction and decreases as the angle increase [1,2]. Therefore, the required energy bandwidth can be obtained only by developing a specific collimation system of the gamma beam, i.e. filtering out the radiation emitted at larger angles. The angular acceptance of the collimation for ELI-NP-GBS must be continuously adjustable in a range from about 700 to 60 μrad, to obtain the required parameters in the entire energy range. The solution identified is a stack of adjustable slits, arranged with a relative rotation around the beam axis to obtain an hole with an approximately circular shape. In this contribution, the final collimation design and its performance evaluated by carrying out a series of detailed Geant4 simulations both of the high-energy and the low-energy beamline are presented

Simulations and Studies of Electron Beam Dynamics under Compton Back-scattering for the Compact X-ray Source ThomX

MOPWO004 - ISBN 978-3-95450-122-9International audienceIn this article are presented beam dynamics investiga- tions of a relativistic electron bunch in the compact storage ring ThomX (50 MeV), which is under construction at LAL to produce hard X-ray using Compton Back-Scattering (CBS). The effect of CBS has been implemented in a 6D tracking code. In addition to CBS, the influence of lattice non linearities and various collective effects on the flux of scattered Compton photons is investigated

Titanium Nitride Coating of RF Ceramic Windows by Reactive DC Magnetron Sputtering

International audienceLAL-Orsay is developing an important effort on R&D and technology studies on RF power couplers for superconductive cavities. These are complex and high technology devices due to their basic functions: vacuum and temperature separation form the environment to the cavity. One of the most critical components of high power couplers is the ceramic RF window that allows the power flux to be injected in the coaxial line. The presence of a dielectric window on a high power RF line has in fact a strong influence on the multipactor phenomena, a resonant electron discharge that is strongly limiting for the RF components performances. The most important method to reduce the multipactor is to decrease the secondary emission yield of the ceramic window. Due to its low secondary electron emission coefficient, TiN thin film is used as a multipactor suppressor coating on RF ceramic coupler windows. In the framework of the EU program FP6 the LAL-Orsay and the LNL-Legnaro establish a collaboration to develop a coating bench that takes into account the different strong constraints on stoechiometry and film coating thickness given by coupler operating conditions. Reactive magnetron sputtering technology was chosen to obtain such deposit. A full description of a sputtering bench recently installed in LAL, and its main characteristics are given. Stoechiometric TiN films are obtained by optimization of reactive gas flow (N2), for a given bias and a given ionisation gas flow (Ar). XRD analysis was performed to control film composition. From the data obtained, lattice parameter is calculated for each deposit and film stoechiometry is determined. XPS analysis of stoechiometric film had shown the existence of oxygen and carbon mainly in the surface. However, it shows also that the ratio Ti/N in atomic percentage is equal to 1